文章基本信息

标题：Induction of Apoptosis in Human Leukemia Cell (Jurkat) by Neolignans Isolated from Seeds of Licaria puchury-major
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作者：Taketo Uchiyama ; Keiichi Tabata ; Sawako Nomura 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2009
卷号：32
期号：10
页码：1749-1753
DOI：10.1248/bpb.32.1749
出版社：The Pharmaceutical Society of Japan
摘要：Ethanol extract of the seeds of Licaria puchury-major , a Brazilian herbal medicine, was found to inhibit cell proliferation in human leukemia cell line (Jurkat) by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Bioassay-guided fractionation of the active components led to the isolation of one phenylpropanoid (1) and ten neolignans (2—11). The apoptosis-inducing activity of the compounds showing cytotoxicity in Jurkat cells was assessed by flow cytometric analysis. Among the identified neolignans, compounds 3, 4, 6 and 7 which have similar molecular structures, showed apoptotic activity. To elucidate the mechanism of apoptosis induction by neolignans, intracellular caspase-3, -8 and -9 activity in Jurkat cells was evaluated. Compound 4 markedly elevated the activity of caspase-3 and -9.
关键词：neolignan;apoptosis;Licaria puchury-major;leukemia;caspase