文章基本信息

标题：Clitocybins, Novel Isoindolinone Free Radical Scavengers, from Mushroom Clitocybe aurantiaca Inhibit Apoptotic Cell Death and Cellular Senescence
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作者：Eun-Yi Moon ; Jin-Mi Oh ; Young-Hee Kim 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2009
卷号：32
期号：10
页码：1689-1694
DOI：10.1248/bpb.32.1689
出版社：The Pharmaceutical Society of Japan
摘要：High concentration of intracellular reactive oxygen species (ROS) plays a role in damaging biological systems. We isolated clitocybin A from the culture broth of Clitocybe aurantiaca and then clitocybin B and C derivatives were synthesized from clitocybin A. IMR-90 lung fibroblast cells were pre-treated or post-treated with clitocybin A, B and C to the addition of 100 μ M H₂O₂. These compounds inhibited the level of intracellular reactive oxygen species (ROS) and H₂O₂-induced cell death as judged by hypodiploid cell formation. The inhibitory effect of clitocybins on H₂O₂-induced cell death was comparable to that with N -acetylcysteine (NAC), a well-known ROS scavenger. The inhibition of H₂O₂-induced cell death by clitocybins was mediated by the reduction of caspase 3 and 9 activation, cytochrome c release from mitochondria and the degradation of IκB-α and IκB-β, which could be resulted in the prevention of cellular senescence. It suggests that clitocybins are novel compounds scavenging ROS and protect cells from apoptosis and cellular senescence.
关键词：clitocybin;Clitocybe aurantiaca;apoptosis;cellular senescence;reactive oxygen species