文章基本信息

标题：Current Application and Technology of Functional Multineuron Calcium Imaging
本地全文：下载
作者：Shigehiro Namiki ; Yuji Ikegaya
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2009
卷号：32
期号：1
页码：1-9
DOI：10.1248/bpb.32.1
出版社：The Pharmaceutical Society of Japan
摘要：Here we describe recent applications and technical advancements of functional multineuron calcium imaging (fMCI), which monitors the firing activity of more than a thousand neurons through their somatic Ca²⁺ signals. fMCI is used for analysis of various neural circuits under normal and pathological conditions. In vitro fMCI is made more sophisticated by using multipoint illumination and scanning technology with spinning-disk and low-laser-intensity imaging, electron-multiplying charge-coupled device cameras, etc . In vivo fMCI is still developing. We review optical technologies for fast scanning imaging, deep tissue imaging, and recording from moving animals.
关键词：calcium imaging;functional multineuron calcium imaging;large-scale recording;scanning;spinning disk