文章基本信息

标题：Inhibitory Effects of Salvianolic Acid B on the High Glucose-Induced Mesangial Proliferation via NF-κB-Dependent Pathway
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作者：Pei Luo ; Zhenghuai Tan ; Zhifeng Zhang 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2008
卷号：31
期号：7
页码：1381-1386
DOI：10.1248/bpb.31.1381
出版社：The Pharmaceutical Society of Japan
摘要：Salvianolic acid B (Sal B) is one of the major water-soluble compounds isolated from Radix Salviae Miltiorrhizae (Danshen in Chinese) that has been reported to be beneficial to treatment of diabetic complications. However, the mechanisms involved in these effects are not discussed in relation to mesangial proliferation via modulation of NF-κB. To explain this, human mesangial cells were pretreated with or without Sal B (0.1, 1, 10 μ M ) for 24 h and stimulated with high glucose (30 m M ). Then the effects of Sal B on mesangial cells proliferation, extracellular matrix production and the possible mechanisms were evaluated by methylthiazoletetrazolium assay, flow cytometry assay, enzyme-linked immunosorbent assay, gelatin zymography assay and western blot assay. These results indicated that Sal B could inhibit high glucose-induced mesangial cells proliferation and extracellular matrix production in a dose-dependent manner, partially through modulating the cell-cycle progress and MMP-2 and MMP-9 activities via suppressing NF-κB activation, suggesting that Sal B may be a promising agent for treating diabetic nephropathy.
关键词：salvianolic acid B;high glucose;proliferation;NF-κB