文章基本信息

标题：Transport of Prostaglandin E1 across Rat Erythrocyte Membrane
本地全文：下载
作者：Takanori Taogoshi ; Junya Nagai ; Ryoko Yumoto 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2008
卷号：31
期号：6
页码：1288-1291
DOI：10.1248/bpb.31.1288
出版社：The Pharmaceutical Society of Japan
摘要：In this study erythrocyte transport of prostaglandin E₁ (PGE₁) was investigated by employing inside-out membrane vesicles prepared from rat erythrocytes. The uptake of [³H]PGE₁ in the presence of ATP was significantly higher than that of AMP, suggesting the involvement of an ATP-dependent efflux system in PGE₁ transport across the erythrocyte membrane. Coincubation of glutathione with ATP further stimulated the uptake of [³H]PGE₁. The uptake of [³H]PGE₁ in the presence of ATP and glutathione was temperature-sensitive, and various eicosanoids including PGE₂ and PGF_2α decreased the uptake. Multidrug resistance-associated protein (MRP) 4 substrates/inhibitors including methotrexate, indomethacin, taurocholic acid and indocyanine green significantly inhibited [³H]PGE₁ uptake. Western blot analysis revealed that Mrp4 is expressed in rat erythrocyte membrane. These results suggest that the release of PGE₁ from the erythrocyte into the blood circulation may be mediated by ATP-dependent efflux pump(s) such as Mrp4.
关键词：prostaglandin E1;multidrug resistance-associated protein;rat erythrocyte