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标题：Sesquiterpene Furan Compound CJ-01, a Novel Chitin Synthase 2 Inhibitor from Chloranthus japonicus SIEB.
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作者：Nam Hui Yim ; Eui Il Hwang ; Bong Sik Yun 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2008
卷号：31
期号：5
页码：1041-1044
DOI：10.1248/bpb.31.1041
出版社：The Pharmaceutical Society of Japan
摘要：A novel sesquiterpene furan compound CJ-01 was isolated from the methanol extract of the whole plant of Chloranthus japonicus S IEB . by monitoring the inhibitory activity of chitin synthase 2 from Saccharomyces cerevisiae . Based on spectroscopic analysis, the structure of compound CJ-01 was determined as 3,4,8a-trimethyl-4a,7,8,8a-tetrahydro-4a-naphto[2,3-b]furan-9-one. The compound inhibited chitin synthase 2 of Saccharomyces cerevisiae in a dose-dependent manner with an IC₅₀ of 39.6 μg/ml, whereas it exhibited no inhibitory activities against chitin synthase 1 and 3 of S. cerevisiae up to 280 μg/ml. CJ-01 has 1.7-fold stronger inhibitory activity than polyoxin D (IC₅₀=70 μg/ml), a well-known chitin synthase inhibitor. These results indicate that the compound is a specific inhibitor of chitin synthase 2 from S. cerevisiae . In addition, CJ-01 showed antifungal activities against various human and phytopathogenic fungi. Therefore, the compound might be an interesting lead to develop effective antifungal agents.
关键词：fungal cell wall;chitin synthase 2 inhibitor;Chloranthus japonicus;sesquiterpene furan compound CJ-01;antifungal activity