文章基本信息

标题：Re-evaluation of the Antioxidant Prenylated Flavonoids from the Roots of Sophora flavescens
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作者：Hyun Ah Jung ; Da-Mi Jeong ; Hae Young Chung 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2008
卷号：31
期号：5
页码：908-915
DOI：10.1248/bpb.31.908
出版社：The Pharmaceutical Society of Japan
摘要：The objective of this research was to re-evaluate the antioxidant effects of the prenylated flavonoids from Sophora flavescens via in vitro 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2′-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS), peroxynitrite (ONOO⁻), and total reactive oxygen species (ROS) assays. In addition, a further examination of kuraridinol, kurarinol, and kurarinone, also isolated from S. flavescens , was carried out by the inhibition of tert -butylhydroperoxide ( t -BHP)-induced intracellular ROS generation and t -BHP-induced activation of nuclear factor-kappaB (NF-κB). Upon re-examination of the ethyl acetate (EtOAc) soluble fraction of S. flavescens , two major prenylated chalcones, including kuraridin and kuraridinol, along with a minor prenylated flavonol, kushenol C, were isolated as good DPPH scavengers. This was in contrast to the prenylated flavanones, sophoraflavanone G and kurarinone, which were isolated from the methylene chloride (CH₂Cl₂) fraction of the same source. Five flavanones consisting of kushenol E, leachianone G, kurarinol, sophoraflavanone G, and kurarinone exhibited significant antioxidant potentials in the ABTS, ONOO⁻, and total ROS assays; however, the prenylated chalcones and prenylated flavonol showed more potent scavenging/inhibitory activities than the prenylated flavanones. Therefore, the prenylated chalcones and prenylated flavonol, rather than the prenylated flavanones, may make important contributions toward the marked antioxidant capacities of S. flavescens . Furthermore, kuraridinol, kurarinol, and kurarinone showed significant inhibitory activities against intracellular ROS levels as well as NF-κB activation by t -BHP. Overall, the results indicate that S. flavescens and its prenylated flavonoids may possess good anti-inflammatory activity, which is implicated in their significant antioxidant activity.
关键词：prenylated flavonoid;Sophora flavescens;free radical;peroxynitrite;reactive oxygen species;nuclear factor-kappaB