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标题：L-Arginine Protects from Pringle Manoeuvere of Ischemia-Reperfusion Induced Liver Injury
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作者：Pronobesh Chattopadhyay ; Navneet Verma ; Anurag Verma 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2008
卷号：31
期号：5
页码：890-892
DOI：10.1248/bpb.31.890
出版社：The Pharmaceutical Society of Japan
摘要：Pringle described a new technique to reduce blood loss during liver surgery. Adult Wistar rats were subjected to 1 h of partial liver ischemia and followed by 3 h reperfusion. Eighteen Wistar rats were divided into sham-operated control group (I) ( n =6), ischemia and reperfusion (I/R) group (II) ( n =6), L -arginine treated group (100 mg/kg body weight/daily by oral route for 7 d before induced ischemia reperfusion maneuver) (III) ( n =6). Ischemic and reperfusion hepatocellular injury occurred as indicated by increased-alanine transaminase (ALT), aspartate transaminase (AST). Pre-treatment with L -arginine significantly decreased serum-ALT, AST after 1 h ischemia followed by 3 h of reperfusion. Nitric oxide production, in hepatocytes was increased 2 fold and MDA levels significantly decreased by L -arginine treatment as compared to I/R rat. Histopathology and TEM studies showed markedly diminished hepatocellular injury in L -arginine pretreated rats during the hepatic I/R, which reached a level comparable to saline-treated rat of sham operated group. Thus, findings it may be concluded that L -arginine afforded significant protection from hepatobiliary function from I/R injury by nitric oxide production.
关键词：Pringle manoeuvere;ischemic reperfusion injury;nitric oxide;hepatocellular injury; L -arginine