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标题：Generation of Mouse Monoclonal Antibody against (−)-Epigallocatechin Gallate
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作者：Takashi Kuzuhara ; Daisuke Kise ; Yayoi Shirakawa 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2008
卷号：31
期号：5
页码：816-819
DOI：10.1248/bpb.31.816
出版社：The Pharmaceutical Society of Japan
摘要：Green tea is an acknowledged cancer preventive in Japan, and the main constituent of green tea catechins is (−)-epigallocatechin gallate (EGCG). To investigate the bioavailability of EGCG in humans, we generated a monoclonal antibody against EGCG in BALB/c mice by immunizing thyroglobulin-conjugated EGCG. Out of 32 hybridoma cell lines, three hybridomas were selected by enzyme-linked immunosorbent assay (ELISA), and then determined by surface plasmon resonance assay: One hybridoma TG38 produced a specific monoclonal antibody against EGCG. The primary structure of TG38 light chain was then deduced from DNA sequence of the light chain gene. The NCBI-BLAST search showed the uniqueness of TG38 monoclonal antibody, and three amino acid residues specific for TG38 were aligned on two loops and one β-sheet of the tertiary structure of the antibody. The TG38 antibody is the first monoclonal antibody against EGCG and catechins, since it bound to four green tea catechins with a galloyl group.
关键词：green tea catechin;(−)-epigallocatechin gallate;monoclonal antibody;surface plasmon resonance assay;galloyl group;enzyme-linked immunosorbent assay