文章基本信息

标题：Properties and Expression of a Multidrug Efflux Pump AcrAB-KocC from Klebsiella pneumoniae
本地全文：下载
作者：Dai-Wei Li ; Motoyasu Onishi ; Takanori Kishino 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2008
卷号：31
期号：4
页码：577-582
DOI：10.1248/bpb.31.577
出版社：The Pharmaceutical Society of Japan
摘要：We previously reported that we had cloned genes responsible for multidrug resistance from the chromosomal DNA of Klebsiella pneumoniae MGH78578 using a drug-hypersusceptible Escherichia coli strain as a host. One of the recombinant plasmids pETV6 conferred resistance to host cells against a wide range of antimicrobial agents, dyes and detergents. It was revealed that this plasmid carried the acrB_Kp gene and a part of the acrA_Kp gene coding for a multidrug efflux pump belonging to the RND family. We cloned the whole acrA_KpB_Kp operon of K. pneumoniae and characterized the pump. The AcrAB pump utilized TolC as an outer membrane component in cells of E. coli. Elevated energy-dependent efflux of ethidium was observed with cells possessing AcrA_Kp B_Kp-TolC. We cloned a gene coding for an ortholog of TolC from chromosomal DNA of K. pneumoniae , and designated it kocC . It seems that the AcrA_KpB_Kp-KocC complex functions as a potent multidrug efflux pump in K. pneumoniae . We observed a higher level of expression of acrA_Kp in K. pneumoniae MGH78578, a multidrug resistant strain, compared with ATCC10031, a drug susceptible strain.
关键词：multidrug efflux pump;AcrAB-KocC;resistance nodulation cell-division;drug resistance;Klebsiella pneumoniae