文章基本信息

标题：Functional Gene Cloning and Characterization of the SsmE Multidrug Efflux Pump from Serratia marcescens
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作者：Yusuke Minato ; Fereshteh Shahcheraghi ; Wakano Ogawa 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2008
卷号：31
期号：3
页码：516-519
DOI：10.1248/bpb.31.516
出版社：The Pharmaceutical Society of Japan
摘要：We cloned a gene responsible for multidrug resistance from chromosomal DNA of Serratia marcescens , and determined the nucleotide sequence. We designated the gene as ssmE . The deduced amino acid sequence of SsmE showed high similarity with the small multidrug resistance (SMR)-type multidrug efflux pumps. Cells of Escherichia coli KAM32, a drug hyper-susceptible mutant, transformed with a plasmid pESM437 carrying the ssmE gene showed elevated minimum inhibitory concentrations of structurally unrelated antimicrobial agents. E. coli KAM32/pESM437 showed elevated energy dependent efflux of ethidium. Reverse transcriptase polymerase chain reaction (RT-PCR) analysis revealed that ssmE was expressed in cells of S. marcescens .
关键词：multidrug efflux pump;SsmE;drug resistance;Serratia marcescens