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标题：Endocrine Disruptive Effects of Chemicals Eluted from Nitrile-Butadiene Rubber Gloves Using Reporter Gene Assay Systems
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作者：Kanako Satoh ; Ryouichi Nonaka ; Ken-ichi Ohyama 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2008
卷号：31
期号：3
页码：375-379
DOI：10.1248/bpb.31.375
出版社：The Pharmaceutical Society of Japan
摘要：Disposable gloves made of nitrile-butadiene rubber (NBR) are used for contact with foodstuffs rather than polyvinyl chloride gloves containing di(2-ethylhexyl)phthalate (DEHP), because endocrine-disruptive effects are suspected for phthalate diesters including DEHP. However, 4,4′-butylidenebis(6- t -butyl- m -cresol) (BBBC), 2,4-di- t -butylphenol, and 2,2,4-trimetyl-1,3-pentanediol diisobutyrate can be eluted from NBR gloves, and possibly also detected in food. In this study, we examined the endocrine-disrupting effects of these chemicals via androgen receptor (AR) and estrogen receptor (ER)-mediated pathways using stably transfected reporter gene cell lines expressing AR (AR-EcoScreen system) and ER (MVLN cells), respectively. We also examined the binding activities of these chemicals to AR and ER. The IC₅₀ value of BBBC for antagonistic androgen was in the range of 10⁻⁶ M . The strength of inhibition was about 5 times that of a known androgen antagonist, 1,1′-(2,2-dichloroethylidene)bis[4-chlorobenzene] ( p , p ′-DDE), and similar to that of bisphenol A. The IC₅₀ value of BBBC for antagonistic estrogen was in the range of 10⁻⁶ M . These results suggest that BBBC and its structural homologue, 4,4′-thiobis(6- t -butyl- m -cresol) are androgen and estrogen antagonists. It is therefore necessary to study these chemicals in vivo , and clarify their effect on the endocrine system.
关键词：4,4′-butylidenebis(6-t-butyl-m-cresol);androgen antagonist;endocrine disruption;anti-androgenic effect;estrogen antagonist;4,4′-thiobis(6-t-butyl-m-cresol)