文章基本信息

标题：Determination of Albumin in Bronchoalveolar Lavage Fluid by Flow-Injection Fluorometry Using Chromazurol S
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作者：Takaji Sato ; Yoshihiro Saito ; Masahiko Chikuma 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2008
卷号：31
期号：3
页码：336-339
DOI：10.1248/bpb.31.336
出版社：The Pharmaceutical Society of Japan
摘要：A highly sensitive flow injection fluorometry for the determination of albumin was developed and applied to the determination of albumin in human bronchoalveolar lavage fluids (BALF). This method is based on binding of chromazurol S (CAS) to albumin. The calibration curve was linear in the range of 5—200 μg/ml of albumin. A highly linear correlation ( r =0.986) was observed between the albumin level in BALF samples ( n =25) determined by the proposed method and by a conventional fluorometric method using CAS (CAS manual method). The IgG interference was lower in the CAS flow injection method than in the CAS manual method. The albumin level in BALF collected from healthy volunteers ( n =10) was 58.5±13.1 μg/ml. The albumin levels in BALF samples obtained from patients with sarcoidosis and idiopathic pulmonary fibrosis were increased. This finding shows that the determination of albumin levels in BALF samples is useful for investigating lung diseases and that CAS flow injection method is promising in the determination of trace albumin in BALF samples, because it is sensitive and precise.
关键词：flow-injection fluorometry;albumin;chromazurol S;bronchoalveolar lavage fluid