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标题：A Novel Consensus Phosphorylation Motif in Sulfatide- and Cholesterol-3-sulfate-Binding Protein Substrates for CK1 in Vitro
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作者：Fumitaka Kawakami ; Kanzo Suzuki ; Kenzo Ohtsuki 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2008
卷号：31
期号：2
页码：193-200
DOI：10.1248/bpb.31.193
出版社：The Pharmaceutical Society of Japan
摘要：A novel phosphorylation motif for casein kinase 1 (CK1) in response to two sulfated lipids [sulfatide and cholesterol-3-sulfate (SCS)] was determined, using three functional proteins [myelin basic protein (MBP), tau protein (TP) and RhoA (a small GTPase)] and five synthetic MBP peptides as phosphate acceptors for the kinase in vitro . It was found that (i) MBP, p8 (positions 38—118) cleaved from MBP, and a synthetic peptide M103 were effectively phosphorylated by CK1δ in the presence of SCS; (ii) sulfatide in comparison with CH-3S highly enhanced autophosphorylation of CK1δ; (iii) SCS had a high binding affinity with MBP and peptide M103, but not other MBP peptides lacking K-G-R; and (iv) a novel consensus phosphorylation motif (K/R-X-K/R-X-X-S/T) for CK1 was identified among several SCS-binding proteins (SCS-BPs) and three CK1 isoforms (δ, ε and γ). The binding of SCS to two basic brain proteins (MBP and TP) resulted in the high stimulation of their phosphorylation by three CK1 isoforms (α, δ and ε), but not CK1γ. In contrast, an acidic protein (RhoA) was effectively phosphorylated by CK1δ in the presence of SCS, and also highly phosphorylated by CK1γ in the presence of sulfatide. Our results presented here suggest that (i) sulfatide may function as an effective stimulator for autophosphorylation of CK1; and (ii) cellular SCS-binding proteins, containing novel phosphorylation motifs for CK1, may be preferentially phosphorylated by CK1 with isoform specificity at the highly accumulated level of SCS in the brain.
关键词：casein kinase 1;myelin basic protein;tau protein;RhoA;sulfatide;cholesterol-3-sulfate