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标题：Genotoxic and Cytotoxic Effects Produced by Acetogenins Obtained from Annona cherimolia MILL.
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作者：Karol Karla García-Aguirre ; Luis Gerardo Zepeda-Vallejo ; Eva Ramón-Gallegos 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2008
卷号：31
期号：12
页码：2346-2349
DOI：10.1248/bpb.31.2346
出版社：The Pharmaceutical Society of Japan
摘要：In the present study we extracted three isomeric acetogenins (Ace) from the seeds of Annona cherimolia M ILL . (Annonaceae) and determined their genotoxic and cytotoxic potential in mice. Our results showed a significant increase in the rate of micronucleated polychromatic erythrocytes induced by Ace with respect to the value for the control group; the effect was less pronounced than that observed with daunorubicin (Dau). To evaluate cytotoxicity, we determined the proportion of polychromatic erythrocytes with respect to the number of normochromatic erythrocytes, and we found an inhibitory effect induced by Ace that was quite similar to that observed with Dau. Besides, by means of the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2 H -tetrazolium bromide test, we also determined the cytotoxicity of Ace in both a normal fibroblast mouse cell line and in a line derived from human colon cancer. In this assay, the strongest decrease in viability corresponded to the malignant cell line. Our results established for the first time the genotoxic capacity of these compounds in vivo , and confirmed their cytotoxic potential in cultured cells.
关键词：acetogenin;cytotoxicity;genotoxicity;micronuclei;Annona cherimolia