文章基本信息

标题：Ephedrae Herba, a Component of Japanese Herbal Medicine Mao-to, Efficiently Activates the Replication of Latent Human Immunodeficiency Virus Type 1 (HIV-1) in a Monocytic Cell Line
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作者：Toru Murakami ; Hideki Harada ; Mary Ann Suico 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2008
卷号：31
期号：12
页码：2334-2337
DOI：10.1248/bpb.31.2334
出版社：The Pharmaceutical Society of Japan
摘要：The persistence of latent human immunodeficiency virus type 1 (HIV-1)-infected cellular reservoirs, despite prolonged treatment with highly active antiretroviral therapy (HAART), represents a major hurdle to virus eradication. In this study, we evaluated the effect of Japanese herbal medicine on the induction of HIV-1 replication in latently infected monocytic cell line, U1, in order to eradicate virus efficiently. We found that Mao-to was able to induce HIV-1 replication either alone or in combination with tumor necrosis factor-alpha (TNF-α). Among the four components of Mao-to, only Ephedrae herba had strong effects in inducing HIV-1 replication. Analysis by Western blotting revealed that Ephedrae herba induced the nuclear translocation of nuclear factor-kappa B (NF-κB). Reporter assay data also showed that Ephedrae herba and, slightly, Mao-to activated the NF-κB promoter, indicating that these herbal agents may induce HIV-1 replication through NF-κB activation. These findings suggest that Mao-to and its component, Ephedrea herba, may be good candidates to augment HAART by inducing the expression of latent HIV-1 with the ultimate goal of eliminating persistent viral reservoirs in individuals infected with HIV-1.
关键词：human immunodeficiency virus type 1;latent infection;Mao-to;Japanese herbal medicine;Ephedrae herba;nuclear factor-kappa B