文章基本信息

标题：β-Ecdysterone Induces Osteogenic Differentiation in Mouse Mesenchymal Stem Cells and Relieves Osteoporosis
本地全文：下载
作者：Lei Gao ; Guoping Cai ; Xiaojun Shi 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2008
卷号：31
期号：12
页码：2245-2249
DOI：10.1248/bpb.31.2245
出版社：The Pharmaceutical Society of Japan
摘要：The effect on bone tissue of β-ecdysterone, a type of ecdysteroid found in many plants, has not been previously investigated. In this study, we found that β-ecdysterone treatment significantly induced alkaline phosphatase (ALP) activity in mesenchymal stem cells in a dose-dependent manner. Real-time polymerase chain reaction (PCR) showed that Runx2, osteocalcin, and type I collagen expression also increased. ICI182780, a specific estrogen receptor antagonist, inhibited the upregulation of ALP activity. Moreover, β-ecdysterone promoted estrogen receptor (ER) reporter gene activity; however, ICI182780 reversed its effect, suggesting that β-ecdysterone has stimulatory effects on osteogenic differentiation via the ER. Furthermore, β-ecdysterone alleviated osteoporosis symptoms in a mouse model without obvious side effects. Therefore β-ecdysterone may be a promising candidate drug for the treatment of osteoporosis.
关键词：β-ecdysterone;mesenchymal stem cell;osteogenic differentiation;estrogen receptor;osteoporosis