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标题：Optimization of Ginsenosides Hydrolyzing β-Glucosidase Production from Aspergillus niger Using Response Surface Methodology
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作者：Jiang-Ning Hu ; Xue-Mei Zhu ; Ki-Teak Lee 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2008
卷号：31
期号：10
页码：1870-1874
DOI：10.1248/bpb.31.1870
出版社：The Pharmaceutical Society of Japan
摘要：To optimize ginsenosides hydrolyzing β-glucosidase production from Aspergillus niger , response surface methodology was carried out in two stages. The Plackett–Burman design was achieved to screen the important variables that influence β-glucosidase production. Among 10 variables (wheat bran, soybean powder, CaCl₂, ginsenosides, KH₂PO₄, MgSO₄, polyethylene glycol (PEG), medium volume, inoculum size, and stirring speed), it was found that wheat bran, KH₂PO₄, and stirring speed had significant effect on β-glucosidase activity due to very low p -values ( p <0.05). Subsequently, wheat bran, KH₂PO₄, and stirring speed were further optimized using central composite design. The optimal β-glucosidase production was predicted to be 4650.14 U/ml with the combination of factors (wheat bran, 34.51 g/l; KH₂PO₄, 1.78 g/l; stirring speed, 161.60 rpm/min). Finally, under optimal fermentation conditions, ginsenoside Rb₁ was converted to Rd and F₂ by A. niger within 10 min. Little compound K was detected at 30 min, and finally F₂ was completely transformed to compound K within 8 h. The putative conversion pathway of Rb₁ by A. niger was Rb₁, Rd, F₂, and compound K.
关键词：Aspergillus niger;β-glucosidase;ginsenoside;response surface methodology