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标题：Determination of Isepamicin in Human Plasma by HPLC with Fluorescence Detection after Derivatization Using 6-Aminoquinolyl-N-hydroxysuccinimidyl-carbamate
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作者：Shin Hosokawa ; Katsunori Nakamura ; Yukiyoshi Fujita 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2008
卷号：31
期号：10
页码：1866-1869
DOI：10.1248/bpb.31.1866
出版社：The Pharmaceutical Society of Japan
摘要：A simple and rapid high-performance liquid chromatographic method has been developed for determination in human plasma of isepamicin (ISP), an aminoglycoside antibiotic agent. After protein precipitation and clean-up procedure to remove lipophilic contaminants, ISP is derivatized pre-column with 6-aminoquinolyl- N -hydroxysuccinimidyl-carbamate for fluorescence detection. Chromatographic separations are achieved using C₁₈ column and mobile phase consisting of 20 m M KH₂PO₄ containing 8 m M triethylamine (pH 7.0) and acetonitrile (78/22, v/v). Amikacin was used as an internal standard. The calibration curve was linear over a concentration range of 0.5—50 μg/ml. The limit of quantification was 0.5 μg/ml. The intra- and inter-day variabilities of ISP were both less than 17.5%. Both derivatives were stable for at least a week at ambient condition. This assay procedure should have useful application in therapeutic drug monitoring of ISP.
关键词：isepamicin;derivatization;aminoglycoside;therapeutic drug monitoring;6-aminoquinolyl-N-hydroxysuccinimidyl-carbamate;high-performance liquid chromatography