文章基本信息

标题：Evaluation of the Establishment of a Tight Junction in Caco-2 Cell Monolayers Using a Pore Permeation Model Involving Two Different Sizes
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作者：Toshinobu Seki ; Sonoko Harada ; Osamu Hosoya 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2008
卷号：31
期号：1
页码：163-166
DOI：10.1248/bpb.31.163
出版社：The Pharmaceutical Society of Japan
摘要：The tight junction formation in Caco-2 cell monolayers was compared after 9 and 13 d of culture. Four different sized paracellular markers were simultaneously applied to the apical side of the monolayers. The transepithelial resistance and permeability coefficient of mannitol for the monolayers cultured for 13 d were 17.4 and 0.095 times those after 9 d, respectively. The tight junction structure developed during that period. The pore permeation model involving two different sizes was used to quantitatively evaluate the paracellular pathways. The results suggest that there were two-different sized (large and small) pathways in the monolayers cultured for 13 d, while there was only a single large pathway in those cultured for 9 d. The small pathway in the monolayers cultured for 13 d might be a major permeation pathway for the paracellular permeation of urea and the equivalent cylindrical pore radius of the small pathway was less than 0.4 nm, suggesting development of the tight junctions after 13 d.
关键词：paracellular transport;Renkin function;Caco-2 cell;permeability;tight junction;diffusion