文章基本信息

标题：Inhibitory Effects of Kurarinol, Kuraridinol, and Trifolirhizin from Sophora flavescens on Tyrosinase and Melanin Synthesis
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作者：Sook Kyung Hyun ; Won-Hee Lee ; Da Mi Jeong 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2008
卷号：31
期号：1
页码：154-158
DOI：10.1248/bpb.31.154
出版社：The Pharmaceutical Society of Japan
摘要：Previously, it was reported that some prenylated flavonoids contained in the dichloromethane fraction of the ethanolic extract of Sophora flavescens , such as kuraridin, sophoraflavanone G, kurarinone, and kushenol F, are tyrosinase inhibitors; however, based on the level of these inhibitors in the extract, its inhibitory effect on tyrosinase activity was higher than expected. This has led us to further investigate other possible constituents that may contribute to the extract's strong inhibitory activity. The results of this study indicate that kurarinol (1), kuraridinol (2), and trifolirhizin (3), from the ethyl acetate fraction of Sophora extract, can inhibit tyrosinase activity. Compared with kojic acid (16.22±1.71 μ M ), compounds 1—3 possessed potent tyrosinase inhibitory activity with IC₅₀ values of 8.60±0.51, 0.88±0.06, and 506.77±4.94 μ M , respectively. These three compounds were further tested for their inhibitory effects on melanogenesis. In cultured B16 melanoma cells, 1—3 markedly inhibited (>50%) melanin synthesis at 50 μ M . This is the first study indicating that 1—3 exert varying degrees of inhibition on tyrosinase-dependent melanin biosynthesis, and therefore, are candidates as skin-whitening agents.
关键词：tyrosinase;Sophora flavescens;kurarinol;kuraridinol;trifolirhizin;melanoma B16 cell