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标题：Neuroprotective Properties of Gallic Acid from Sanguisorbae Radix on Amyloid β Protein (25—35)-Induced Toxicity in Cultured Rat Cortical Neurons
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作者：Ju Yeon Ban ; Ha Thi Thuy Nguyen ; Hee-Ju Lee 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2008
卷号：31
期号：1
页码：149-153
DOI：10.1248/bpb.31.149
出版社：The Pharmaceutical Society of Japan
摘要：Our previous studies reported that methanol extract of Sanguisorbae radix from Sanguisorba officinalis L. (Rosaceae) prevented neuronal cell damage induced by Aβ (25—35) in vitro . The present study was carried out to investigate the effect of gallic acid isolated from Sanguisorbae radix on Aβ (25—35)-induced neurotoxicity using cultured rat cortical neurons. Gallic acid (0.1, 1 μ M ) showed a concentration-dependent inhibition on Aβ (25—35) (10 μ M )-induced apoptotic neuronal death, as assessed by a 3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyl-tetrazolium bromide (MTT) assay and Hoechst 33342 staining. Pretreatment of gallic acid inhibited 10 μ M Aβ (25—35)-induced elevation of cytosolic Ca²⁺ concentration ([Ca²⁺]_c) and generation of reactive oxygen species (ROS), which were measured by fluorescent dyes. Gallic acid also inhibited glutamate release into medium induced by 10 μ M Aβ (25—35), which was measured by HPLC. These results suggest that gallic acid prevents Aβ (25—35)-induced apoptotic neuronal death by interfering with the increase of [Ca²⁺]_c, and then by inhibiting glutamate release and generation of ROS, and that these effects of gallic acid may be partly associated with the neuroprotective effect of Sanguisorbae radix.
关键词：gallic acid;Sanguisorbae radix;amyloid β protein;neuroprotection;cultured neuron