文章基本信息

标题：The Study to Reduce the Hemolysis Side Effect of Puerarin by a Submicron Emulsion Delivery System
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作者：Peng-Fei Yue ; Hai-Long Hai-Long Yuan ; Wei-Feng Zhu 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2008
卷号：31
期号：1
页码：45-50
DOI：10.1248/bpb.31.45
出版社：The Pharmaceutical Society of Japan
摘要：A safe and effective delivery system with a submicron emulsion for puerarin was studied. Puerarin submicron emulsion was prepared by a novel complex-phase inversion-high press homogenization technology. The mechanism to reduce the hemolysis side effect of puerarin was studied by blood cell counts in rabbits. The average diameter, zeta potential and entrapment efficiency of the emulsion prepared was 198.14±8.61 nm, −29.45±1.47 mV, 87.32±0.34%, respectively. Compared with control group, the red blood cell values, packed cell volume, plasma hemoglobin level, haptoglobin level and osmotic fragility of puerarin i.v. group was significantly different ( p <0.05) at 42, 43 d, respectively. The blood cell parameter values of puerarin submicron emulsion group were not significantly different ( p >0.05) in contrast to control group. Such observations indicated that the intravascular hemolysis occurred at 42, 43 d in puerarin i.v. group rabbits, the hemolysis did not occur for puerarin emulsion group rabbits. As an explanation for these results, it was proposed that the puerarin was either incorporated into the lipophilic core or intercalated between the phospholipid molecules at the interface. It could be concluded that puerarin submicron emulsions prepared markedly reduced the hemolysis effect of puerarin.
关键词：puerarin;submicron emulsion;hemolysis;blood cell count