文章基本信息

标题：Determination of Digoxin in Human Serum Using Stable Isotope Dilution Liquid Chromatography/Electrospray Ionization-Tandem Mass Spectrometry
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作者：Kuniko Mitamura ; Aki Horikawa ; Yuko Yamane 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2007
卷号：30
期号：9
页码：1653-1656
DOI：10.1248/bpb.30.1653
出版社：The Pharmaceutical Society of Japan
摘要：A method for the determination of digoxin in human serum using a liquid chromatography/electrospray ionization-tandem mass spectrometry (LC/ESI-MS/MS) technique is reported. Digoxin and the internal standard, [21,21,22-²H₃]digoxin, were extracted from 250 μl of human serum using a solid phase extraction cartridge (Oasis HLB) and analyzed by LC/ESI-MS/MS in the selected reaction monitoring mode. The intra- and inter-assay reproducibility and accuracy were satisfactory within the quantification range of 0.20—3.20 ng/ml. The concentrations of digoxin in the serum samples obtained from digitalized patients ( n =19) were in the range of 0.25—2.84 ng/ml, which were compared to those obtained by radioimmunoassay.
关键词：digoxin;serum;liquid chromatography/tandem mass spectrometry;digitalized patient;correlation;radioimmunoassay