文章基本信息

标题：Blocking Effect of Anti-Dectin-1 Antibodies on the Anti-tumor Activity of 1,3-β-Glucan and the Binding of Dectin-1 to 1,3-β-Glucan
本地全文：下载
作者：Yoshihiko Ikeda ; Yoshiyuki Adachi ; Takashi Ishii 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2007
卷号：30
期号：8
页码：1384-1389
DOI：10.1248/bpb.30.1384
出版社：The Pharmaceutical Society of Japan
摘要：Schizophyllan (SPG) is used to treat cervical cancer in combination with irradiation to enhance the immunological surveillance system. Dectin-1 is a cell surface receptor for 1,3-β-glucan. In this study, we prepared two anti-Dectin-1 monoclonal antibodies, 4B2 and SC30 having a K _D of 7.04×10 ⁻⁸ M and 1.55×10 ⁻⁷ M , respectively, and evaluated the role of Dectin-1 in SPG-induced anti-tumor activity in mice. Expression of Dectin-1 on peritoneal macrophages and binding of SPG to the cells were decreased by administration of 4B2 and SC30. SPG-mediated anti-tumor activity was inhibited by 4B2 and SC30. 4B2 and SC30 inhibited the binding of SPG to splenocytes from mice. The binding of SPG-biotin to Dectin-1-transfected HEK293 cells was inhibited by 4B2, but not SC30. 4B2 and SC30 differ in their influence on Dectin-1 between primary cells and transduced cells, and Dectin-1 effects 1,3-β-glucan-mediated anti-tumor activity in mice by binding to SPG.
关键词：β-glucan;Dectin-1;antitumor activity