文章基本信息

标题：DNA Authentication of Plantago Herb Based on Nucleotide Sequences of 18S–28S rRNA Internal Transcribed Spacer Region
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作者：Fatma Pinar Sahin ; Hiromi Yamashita ; Yahong Guo 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2007
卷号：30
期号：7
页码：1265-1270
DOI：10.1248/bpb.30.1265
出版社：The Pharmaceutical Society of Japan
摘要：Internal transcribed spacer (ITS) regions of nuclear ribosomal RNA gene were amplified from 23 plant- and herbarium specimens belonging to eight Plantago species ( P. asiatica , P. depressa , P. major , P. erosa , P. hostifolia , P. camtschatica , P. virginica and P. lanceolata ). Sequence comparison indicated that these Plantago species could be identified based on the sequence type of the ITS locus. Sequence analysis of the ITS regions amplified from the crude drug Plantago Herb obtained in the markets indicated that all the drugs from Japan were derived from P. asiatica whereas the samples obtained in China were originated from various Plantago species including P. asiatica , P. depressa , P. major and P. erosa .
关键词：Plantago Herb;DNA authentication;internal transcribed spacer;internal transcribed spacer (ITS);ribosomal DNA