文章基本信息

标题：Inhibitory Effect of p-Hydroxybenzyl Alcohol on Tyrosinase Activity and Melanogenesis
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作者：Szu-Hsiu Liu ; I-Horng Pan ; I-Ming Chu 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2007
卷号：30
期号：6
页码：1135-1139
DOI：10.1248/bpb.30.1135
出版社：The Pharmaceutical Society of Japan
摘要：Tyrosinase is a key enzyme catalyzing the rate-limiting step for the biosynthesis pathway of melanin pigment, which is the most important determinant of the color of skin. Inhibiting tyrosinase and repressing melanocyte metabolism can reduce melanin production. Among the possible melanin reducing compounds, tyrosinase inhibitors are most promising for treating pigmentation and are used as skin-whitening agents in the cosmetic industry. In our investigation, some new tyrosinase inhibitors from plants have been identified to have high tyrosinase inhibitory activity. Specifically, p -hydroxybenzyl alcohol (4HBA) was found to inhibit the monophenolase activity of mushroom tyrosinase. When 4HBA binds with the enzyme, conformation of the enzyme is altered and its activity decreases. The inhibitory effect of 4HBA on melanogenesis has been studied using cultured mouse melanoma cells. Melanin synthesis in cell culture with 4HBA at 1.0 m M was decreased to 45% of control and below 1.0 m M there was no effect on cell growth. The inhibitory effects of 4HBA on melanogenesis are due to the direct inhibition of melanosomal tyrosinase activity, rather than to the suppression of tyrosinase gene. These results showed that 4HBA is a promising and safe agent for skin whitening.
关键词：tyrosinase;melanogenesis;melanin;p-hydroxybenzyl alcohol;mouse melanoma cell