文章基本信息

标题：Contribution of Ca2+-Dependent Protein Kinase C in the Spinal Cord to the Development of Mechanical Allodynia in Diabetic Mice
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作者：Kenji Honda ; Masako Koguchi ; Kohei Koga 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2007
卷号：30
期号：5
页码：990-993
DOI：10.1248/bpb.30.990
出版社：The Pharmaceutical Society of Japan
摘要：In this paper, we directly demonstrate, for the first time, the activation of Ca²⁺-dependent protein kinase C (PKC) in the spinal cord of diabetic mice. In streptozotocin (STZ)-treated (200 mg/kg, i.v.) diabetic mice, hypersensitivity (allodynia) to mechanical stimulation appeared 7 d after STZ injection. This mechanical allodynia was inhibited by intrathecal injection of the PKC inhibitors 1-(5-isoquinolinesulfonyl)-2-methylpiperazine (H-7) and calphostin C, but not the protein kinase A inhibitor N -[2-( p -bromocinnamylamino)ethyl]-5-isoquinolinesulfonamide (H-89). The activity of membrane-associated Ca²⁺-dependent PKC in the spinal cords of STZ-induced diabetic mice was significantly higher than that observed in non-diabetic mice. These results suggest that activation of Ca²⁺-dependent PKC in the spinal cord, contributes to the mechanical allodynia in the pain associated with diabetic neuropathy.
关键词：diabetic mice;mechanical allodynia;spinal cord;Ca2+-dependent protein kinase C