文章基本信息

标题：Mouse Equilibrative Nucleoside Transporter 2 (mENT2) Transports Nucleosides and Purine Nucleobases Differing from Human and Rat ENT2
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作者：Katsuhito Nagai ; Kazuki Nagasawa ; Yoji Kyotani 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2007
卷号：30
期号：5
页码：979-981
DOI：10.1248/bpb.30.979
出版社：The Pharmaceutical Society of Japan
摘要：Several mammalian nucleoside transporters have been identified at the molecular level. Human and rat equilibrative nucleoside transporter 2 (hENT2 and rENT2, respectively) was previously reported to have the dual ability of transporting both nucleosides and nucleobases. In the present study, we characterized the transport of a variety of nucleosides and nucleobases via recombinant mouse ENT2 (mENT2). Cloned mENT2 mediated the uptake of nucleosides and purine nucleobases, but not pyrimidine nucleobases. The mENT2-mediated uptake of adenosine was significantly inhibited by nucleosides and nucleobases, irrespective of purine and pyrimidine. The K _m values for the uptake of nucleosides and purine nucleobases mediated by mENT2 varied between 1.24 and 16.3 μ M , and the transport clearances of adenosine and hypoxanthine via the transporter were greater than those of other substrates. Therefore, we concluded that mENT2 is nucleoside and purine nucleobase transporter, and pyrimidine nucleobases are blockers for the transporter, differing from hENT2 and rENT2 that were reported to also transport pyrimidine nucleobases.
关键词：nucleoside;nucleobase;nucleoside transporter;ENT2;chemotherapy