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标题：Increase in Intracellular Ca2+ Concentrations and the Corresponding Intracellular Acidification Are Early Steps for Induction of Apoptosis by Geranylgeraniol in HL60 Cells
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作者：Yutaka Masuda ; Toshihiro Aiuchi ; Saori Mihara 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2007
卷号：30
期号：5
页码：880-884
DOI：10.1248/bpb.30.880
出版社：The Pharmaceutical Society of Japan
摘要：To elucidate the mechanism of induction of apoptosis by geranylgeraniol (GGO), which is a potent inducer of apoptosis in various lines of human cancer cells, we examined the role of intracellular acidification during GGO-induced apoptosis using human leukemia HL60 cells. Flow cytometry analysis revealed that apoptosis induced in human leukemia HL60 cells by GGO was associated with intracellular acidification. Both GGO-induced intracellular acidification and apoptosis as analyzed by DNA fragmentation were inhibited by phorbol myristate acetate (TPA) and O ′-bis(2-aminophenyl)ethyleneglycol- N , N , N ′, N -tetraacetic acid tetraacetoxymethyl ester (BAPTA-AM), an intracellular Ca²⁺ chelator, but not by ethyleneglycol-bis(2-aminoethylether)- N , N , N ′, N ′-tetraacetic acid (EGTA). These results suggest that the early concentration change of intracellular Ca²⁺ and the corresponding decrease in intracellular pH are required for the induction of apoptosis in HL60 cells by GGO.
关键词：geranylgeraniol;apoptosis;acidification;calcium ion;leukemia cell;HL60