文章基本信息

标题：Extracellular ATP Is Involved in the Induction of Apoptosis in Murine Hematopoietic Cells
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作者：Mi-Jung Yoon ; Hyun-Joo Lee ; Yong-Soo Lee 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2007
卷号：30
期号：4
页码：671-676
DOI：10.1248/bpb.30.671
出版社：The Pharmaceutical Society of Japan
摘要：Extracellular nucleotides have multiple biological actions in processes such as proliferation, differentiation, chemotaxis, and cytokine secretion through P2X receptors on the cell surface. To determine the biological activity of adenosine triphosphate (ATP) and the expression of P2 nucleotide receptors in murine bone marrow-derived hematopoietic cells and stem cells/progenitor cells, we investigated the effects of ATP in assays of cell proliferation and cell death in vitro . Our results demonstrated that several subtypes of P2X receptors were expressed on hematopoietic cells and that P2X₇, in particular, was partially expressed in hematopoietic stem cells/progenitor cells. In addition, stimulation of hematopoietic cells with high concentrations of ATP caused severe inhibition of cell proliferation despite the presence of cytokine stimulation. We analyzed the apoptotic effects of stimulation with several different dosages of ATP and confirmed the enhanced apoptotic activity in hematopoietic cells and progenitor cells. Antagonists, against P2X receptors and ATP, suramin and oxidized ATP, inhibited the induction of cell death for murine hematopoietic cells. Our data suggest that extracellular nucleotides may provide a novel and powerful tool for regulating the cell fate of hematopoietic stem cells.
关键词：ATP;hematopoietic stem cell;P2X;proliferation;apoptosis;antagonis