文章基本信息

标题：Molecular Cloning and Characterization of a Multidrug Efflux Pump, SmfY, from Serratia marcescens
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作者：Fereshteh Shahcheraghi ; Yusuke Minato ; Jing Chen 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2007
卷号：30
期号：4
页码：798-800
DOI：10.1248/bpb.30.798
出版社：The Pharmaceutical Society of Japan
摘要：We cloned a gene smfY for multidrug efflux pump from chromosomal DNA of Serratia marcescens using drug-hypersensitive Escherichia coli KAM32 as the host, and characterized the pump. E. coli KAM32/pESM42 carrying the smfY showed significantly increased MICs of various drugs including DAPI, norfloxacin, benzalkonium chloride, acriflavine and ethidium bromide, compared with the control. We also detected energy-dependent ethidium and acriflavine efflux due to the SmfY. Sequence analysis revealed that the SmfY was a multidrug efflux pump of the MF (Major Facilitator) superfamily transporters. This is the first report of a multidrug efflux pump belonging to the MF superfamily in S. marcescens .
关键词：multidrug efflux pump;SmfY;major facilitator (MF) superfamily;Serratia marcescens