文章基本信息

标题：Discrimination of Lonicera japonica THUNB. from Different Geographical Origins Using Restriction Fragment Length Polymorphism Analysis
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作者：Chong-Zhi Wang ; Ping Li ; Jia-Yi Ding 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2007
卷号：30
期号：4
页码：779-782
DOI：10.1248/bpb.30.779
出版社：The Pharmaceutical Society of Japan
摘要：Lonicera japonica T HUNB . is a commonly used anti-inflammatory herbal medicine. The therapeutic effectiveness of L. japonica depends significantly on its geographical origin. However, it is difficult to define criteria for confirming geographical authenticity using microscopic and chemical characteristics. In the present study, the internal transcribed spacer (ITS) region of the nuclear ribosomal DNA loci of L. japonica from different origins and related species was sequenced. The mutation site found in the ITS region from geo-authentic L. japonica can be recognized by the restriction endonuclease Eco N I. Since PCR products from geo-authentic L. japonica cannot be digested completely, a quantitative restriction fragment length polymorphism analysis method was developed. The cleavage rate of PCR products by Eco N I was determined to be more than 70% in all geo-authentic L. japonica and less than 20% in non-geo-authentic L. japonica and other species from genus Lonicera . The rate correlated remarkably with the geographical origin of L. japonica . Therefore, this method can be used to classify geo-authentic L. japonica .
关键词：Lonicera japonica;geographical origin;authentication;restriction fragment length polymorphism