文章基本信息

标题：Adhesion of Candida albicans to HeLa Cells: Studies Using Polystyrene Beads
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作者：Masayoshi Miyauchi ; Philippe Giummelly ; Shin Yazawa 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2007
卷号：30
期号：3
页码：588-590
DOI：10.1248/bpb.30.588
出版社：The Pharmaceutical Society of Japan
摘要：The adherence to HeLa cells by the yeast-type cells of the Candida albicans NIH A-207 strain cultivated for 2 d at 27 °C in the yeast extract-added Sabouraud liquid medium (YSLM) and the 500 m M galactose-added yeast nitrogen base medium (YNB-Gal) was compared. The yeast cells cultured in the YNB-Gal clearly increased the adherence to the HeLa cells compared to the YSLM. The adherence drastically decreased by pronase treatment of the yeast cells. Next, to define the sugar receptors of the yeast cells, lactosylceramide (LC)-, the H type 1 antigen (HA)-, Lewis^a antigen (Le^a)-, mannan- and BSA-coated polystyrene beads were used for the adherence to the yeast cells. Only the LC- and HA-beads were bound to the yeast cells. The adherence of the two beads to the yeast cells cultured in the YNB-Gal was higher than that to the yeast cells cultured in the YSLM. The yeast cell wall mannan-coated polystyrene beads did not adhere at all to the Hela cells. Based on these results, it is evident that the protein parts involving the LC and HA receptors on the surface of the yeast cells correlate with the adherence to the HeLa cells.
关键词：Candida albicans;HeLa cell;adherence;polystyrene bead;sugar receptor;mannan