文章基本信息

标题：Checkpoint Kinase 1 Is Cleaved in a Caspase-Dependent Pathway during Genotoxic Stress-Induced Apoptosis
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作者：Naoyuki Okita ; Yuki Kudo ; Sei-ichi Tanuma 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2007
卷号：30
期号：2
页码：359-362
DOI：10.1248/bpb.30.359
出版社：The Pharmaceutical Society of Japan
摘要：Checkpoint kinase 1 (Chk1) plays important roles in genotoxic stress-induced cell cycle checkpoint and in normal cell cycle progression. Here, we show that Chk1 is cleaved in the treatment of apoptotic dose of etoposide (ETP) or cisplatin (CIS) but not of viable dose in HeLa S3 cells. The cleavage of Chk1 was completely inhibited by an irreversible and cell-permeable pan-caspase inhibitor, N -benzyloxycarbonyl-Val-Ala-Asp (OMe) fluoromethylketone (z-VAD-fmk). These results identify Chk1 as a novel substrate that is cleaved by a caspase-dependent manner during genotoxic stress-induced apoptosis. Our data may also indicate the existence of a novel Chk1-regulated apoptotic pathway.
关键词：checkpoint kinase 1 (Chk1);genotoxic stress;cell cycle checkpoint;DNA repair;apoptosis;caspase