文章基本信息

标题：Induction of Apoptosis by the Licochalcone E in Endothelial Cells via Modulation of NF-κB and Bcl-2 Family
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作者：Hee Jung Chang ; Goo Yoon ; Jung Sun Park 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2007
卷号：30
期号：12
页码：2290-2293
DOI：10.1248/bpb.30.2290
出版社：The Pharmaceutical Society of Japan
摘要：Licochalcones have a variety of biological properties including anti-tumor, anti-parasitic and anti-bacterial activities. Recently, a new retrochalcone (licochalcone E, Lico-E) was isolated from the roots of Glycyrrhiza inflata ( Chem. Pharm. Bull. , 53, 2005, Yoon et al. ) by cytotoxicity-guided fractionation. This study examined whether or not Lico-E-induced endothelial cell death occurs through apoptosis, and investigated molecular mechanisms involved in this process. Lico-E was found to suppress ECV304 cell growth and induce apoptosis. The induction of apoptosis by Lico-E was confirmed by the ladder-patterned DNA fragmentation, the presence of cleaved and condensed nuclear chromatin and the increased number of annexin V-positive cells. Lico-E could effectively inhibit the constitutive NF-κB activation, as revealed by the electrophoretic mobility shift assay and NF-κB-dependent luciferase reporter study. In addition, the Lico-E treatment caused a change in the Bax/Bcl-2 ratio that favored apoptosis. These results suggest that Lico-E induces endothelial cell apoptosis by modulating NF-κB and the Bcl-2 family.
关键词：licochalcone E;endothelia;apoptosis;nuclear factor-κB;Bcl-2