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标题：Morphine Inhibits Erythrocyte Carbonic Anhydrase in Vitro and in Vivo
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作者：Taha Abdulkadir Çoban ; Şükrü Beydemir ; İlhami Gülçin 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2007
卷号：30
期号：12
页码：2257-2261
DOI：10.1248/bpb.30.2257
出版社：The Pharmaceutical Society of Japan
摘要：Morphine is implicated in diverse functions, from development to immune modulation in the central and peripheral nervous systems. At the present time, morphine is one of the most effective antinociceptive agents used to manage pain. It has been used extensively in the clinical management of pain due to its potent analgesic effect. In this study, the in vitro and in vivo inhibitory effects of morphine on erythrocyte carbonic anhydrase (CA) were investigated. Human erythrocyte isoenzymes, HCA-I and HCA-II, were purified by Sepharose-4B affinity chromatography column with a yield of 66.95 and 62.82%, a specific activity of 3892.3 and 11663.2 EU/mg proteins with 745.1 and 2232.6-fold purification of each isoenzyme, respectively. To determine enzyme purity, sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) was performed. In vitro inhibition of erythrocyte HCA-I and HCA-II by morphine using the CO₂-hydratase enzyme gave IC₅₀ values 4.50×10⁻⁵ M ( r ²: 0.954) and 9.23×10⁻⁵ M ( r ²: 0.996), respectively. CA activity was significantly attenuated in vivo in Spraque-Dawley rats for up to 3 h ( p <0.001) following intraperitoneal administration of morphine. In conclusion, morphine inhibited CA activity both in vitro and in vivo .
关键词：carbonic anhydrase;morphine;erythrocyte;inhibition;enzyme