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标题：In Situ Intestinal Absorption Behaviors of Tanshinone IIA from Its Inclusion Complex with Hydroxypropyl-β-cyclodextrin
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作者：Wang Ling ; Li Chen Rui ; Jiang Xue Hua 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2007
卷号：30
期号：10
页码：1918-1922
DOI：10.1248/bpb.30.1918
出版社：The Pharmaceutical Society of Japan
摘要：In this paper, the intestinal permeability of the inclusion complex of tanshinone IIA (TS IIA) with 2-hydroxypropyl-β-cyclodextrin (HP-β-CD) was investigated. The corresponding complexation of TS IIA–HP-β-CD was obtained by coevaporation and characterized by differential scanning calorimetry and X-ray diffraction. The recirculation intestinal perfusion technique in rats was used to study the absorption behavior of free and complexed TS IIA. The change of concentration of TS IIA was separately calculated according to Michaelis–Menten and the Fick's equation to investigate its absorption rate-limiting step. Using the mathematical models above, it was concluded that the limit step to absorption of TS IIA was the dissolution process. Different concentrations of complexed TS IIA were administrated to three intestinal segments, with the intestinal permeability ranging from 3.16×10⁻⁵ cm·s⁻¹ in the duodenum (50 μg·ml⁻¹) to 4.11×10⁻⁵ cm·s⁻¹ in the jejunum (100 μg·ml⁻¹). With the increase of dosage of complex, TS IIA's absorption did not show saturated phenomenon, suggesting its transport mechanism in vivo might primary be passive transport. Besides, the permeability of TS IIA was not apparently influenced by the perfusion section studied, which indicated that there might not exist specific absorption site for TS IIA.
关键词：tanshinone IIA;inclusion complex;hydroxypropyl-β-cyclodextrin;recirculation intestinal perfusion;intestinal absorption