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标题：Component and Antioxidant Properties of Polysaccharide Fractions Isolated from Angelica sinensis (OLIV.) DIELS
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作者：Xingbin Yang ; Yan Zhao ; Yanjuan Zhou 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2007
卷号：30
期号：10
页码：1884-1890
DOI：10.1248/bpb.30.1884
出版社：The Pharmaceutical Society of Japan
摘要：An analytical method of high performance capillary electrophoresis (HPCE) was developed to simultaneously separate and identify the component monosaccharides of Angelica sinensis polysaccharide fractions (APFs), named APF1, APF2 and APF3. The predominant sugars in APFs were identified as arabinose, glucose, rhamnose, galactose and galacturonic acid as well as trace amount of mannose and glucuronic acid, and the fractionation altered significantly the distribution of component monosaccharides in APFs. APF3 was the most active fraction to effectively inhibit H₂O₂-caused decrease of cell viability, lactate dehydrogenase (LDH) leakage and malondialdehyde (MDA) formation, and also reduced H₂O₂-caused decline of superoxide dismutase (SOD) activity and glutathione (GSH) depletion ( p <0.05), followed by APF2 and APF1 in decreasing order. Furthermore, it was found that APFs (100 μg/ml) could protect macrophages by inhibiting the release of excess NO and reactive oxygen species (ROS) induced by high concentrations of H₂O₂ (0.8—1.6 m M ).
关键词：Angelica sinensis;polysaccharide fraction;capillary electrophoresis;macrophage;antioxidant activity