文章基本信息

标题：Gene Cloning and Characterization of KdeA, a Multidrug Efflux Pump from Klebsiella pneumoniae
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作者：Yu Ping ; Wakano Ogawa ; Teruo Kuroda 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2007
卷号：30
期号：10
页码：1962-1964
DOI：10.1248/bpb.30.1962
出版社：The Pharmaceutical Society of Japan
摘要：We cloned a gene related to multidrug resistance from a drug-resistant Klebsiella pneumoniae strain MGH78578. We designated the gene kdeA , which encodes a protein possessing 12 hydrophobic regions. The deduced amino acid sequence of KdeA is similar to that of MdfA, a well-characterized multidrug efflux pump from Escherichia coli . Introduction of the kdeA gene into cells of the drug-hypersusceptible E. coli strain KAM32 resulted in elevated minimum inhibitory concentrations of chloramphenicol, norfloxacin, acriflavine, and ethidium bromide. We observed elevated energy-dependent ethidium efflux activity with cells carrying kdeA compared with control cells. We also observed expression of kdeA in cells of K. pneumoniae under normal growth conditions.
关键词：multidrug resistance;multidrug efflux pump;kdeA;Klebsiella pneumoniae