文章基本信息

标题：Electrospray Ionization and Time-of-Flight Mass Spectrometric Method for Simultaneous Determination of Spermidine and Spermine
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作者：Keijiro Samejima ; Masahiro Otani ; Yasuko Murakami 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2007
卷号：30
期号：10
页码：1943-1946
DOI：10.1248/bpb.30.1943
出版社：The Pharmaceutical Society of Japan
摘要：A sensitive method for the determination of polyamines in mammalian cells was described using electrospray ionization and time-of-flight mass spectrometer. This method was 50-fold more sensitive than the previous method using ionspray ionization and quadrupole mass spectrometer. The method employed the partial purification and derivatization of polyamines, but allowed a measurement of multiple samples which contained picomol amounts of polyamines. Time required for data acquisition of one sample was approximately 2 min. The method was successfully applied for the determination of reduced spermidine and spermine contents in cultured cells under the inhibition of aminopropyltransferases. In addition, a new proper internal standard was proposed for the tracer experiment using ¹⁵N-labeled polyamines.
关键词：polyamine;heptafluorobutyryl derivative;time-of-flight mass spectrometry;electrospray ionization;stable isotope labeled polyamine;cultured mammalian cell