文章基本信息

标题：Tetrandrine Inhibits Proinflammatory Cytokines, iNOS and COX-2 Expression in Human Monocytic Cells
本地全文：下载
作者：Shu-Jing Wu ; Lean-Teik Ng
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2007
卷号：30
期号：1
页码：59-62
DOI：10.1248/bpb.30.59
出版社：The Pharmaceutical Society of Japan
摘要：Tetrandrine (TET), a bis-benzylisoquinoline alkaloid isolated from the dried root of Hang-Fang-Chi ( Stephania tetrandra S. M OORE ), is traditionally used in China for treating inflammation, hypertension and silicosis. In this study, our aim was to examine the anti-inflammatory mechanism of TET through measuring the inducible nitric oxide synthase (iNOS), cyclooxygenase-1, and -2 (COX-1 and COX-2) expression, cytokines (TNF-α, IL-4 and IL-8) formation, nitric oxide (NO) release and prostaglandin E₂ (PGE₂) generation in lipopolysaccharide (LPS)-induced human monocytic (THP-1) cells. Results showed that TET remarkably suppressed the LPS (1 μg/ml) induction of NO release and PGE₂ generation. It also significantly attenuated the LPS-induced transcription of proinflammatory cytokines (TNF-α, IL-4 and IL-8) in a dose-dependent manner. Furthermore, TET at 100 μ M significantly blocked the LPS induction of iNOS and COX-2 expression, but not the COX-1. Taken together, these results suggest that TET exerts anti-inflammatory effects probably through the suppression of COX-2 and iNOS expression.
关键词：tetrandrine;cytokine;inducible nitric oxide synthase (iNOS);COX-2;THP-1 cell