文章基本信息

标题：Protective Effect of Salvianic Acid A on Acute Liver Injury Induced by Carbon Tetrachloride in Rats
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作者：Chao-Yun Wang ; Fu-Lu Ma ; Jun-Tian Liu 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2007
卷号：30
期号：1
页码：44-47
DOI：10.1248/bpb.30.44
出版社：The Pharmaceutical Society of Japan
摘要：Previous research has shown that salvianic acid A [2-(3,4-dihydroxyphenyl)-2-hydroxy-propanoic acid, SA] extracted from Salvia miltiorrhiza B GE (Danshen) markedly inhibits lipid peroxidation of mitochondrial membrane of hepatic cells in vitro . The present study was conducted to examine protective effect of SA on liver injury induced by carbon tetrachloride (CCl₄) and its possible mechanism in vivo . Male Sprague-Dowley rats weighing 180—200 g were used in the experiments. Five mmol/kg CCl₄ in olive oil was given to rats i.p. Spectrophotometrical method was used to measure activities of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) in serum, activities of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) as well as malondialdehyde (MDA) level in hepatic tissue and the rate of superoxide anion (O₂·⁻) generation in hepatic submitochondrial particles. Hepatic histological structure was observed under light microscopy. CCl₄ caused significant changes of activities of the enzymes, MDA level, and the rate of O₂·⁻ generation and histopathological changes of acute hepatic injury were noted. SA reversed the significant changes induced by CCl₄. These results demonstrate that SA produces protective action on acute hepatic injury induced by CCl₄ via an antioxidative mechanism.
关键词：salvianic acid A;aspartate aminotransferase;alanine aminotransferase;antioxidative enzyme;malondialdehyde;superoxide anion