文章基本信息

标题：The Isolation DNA Chromosomal DNA from Local Isolate of Aeromonas hydrophila Bacteria from East Java
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作者：Retno Sri Wahjuni ; M. Gandul Atik Yuliani
期刊名称：Jurnal Vokasi Indonesia
印刷版ISSN：2355-5807
电子版ISSN：2477-3433
出版年度：2021
卷号：9
期号：2
DOI：10.7454/jvi.v6i1.115
语种：English
出版社：Universitas Indonesia
摘要：Aeromoniasis is a bacterial disease caused by Aeromonas hydrophila that affects fish and shrimps in ponds and aquariums. This is an ulcer disease that causes petechiae in scales of fish and may be fatal. It can cause economic loss if it is not treated with medication accompanied with improved sanitation. It affects numerous freshwater fish farms in East Java. A previous study has characterized an antigenic protein derived from the outer membrane protein of A. hydrophila. In order to perform sequencing of the DNA coding for this protein, we first conducted a study for isolating DNA fragments of A. hydrophila via four stages: cultivation and harvesting of bacterial cells, cell lysis, DNA purification, and the concentration of chromosomal DNA. The results will be used as a predictive immunogenic determinant by the method by Kolaskar and Tongaonkar.
关键词：Aeromonas hydrophila; chromosomal DNA; immunogenic determinant