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标题：Suppression of the Biosynthesis of Cellular Sphingolipids Results in the Inhibition of the Maturation of Influenza Virus Particles in MDCK Cells
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作者：Kazuya I. P. J. Hidari ; Yasuo Suzuki ; Takashi Suzuki 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2006
卷号：29
期号：8
页码：1575-1579
DOI：10.1248/bpb.29.1575
出版社：The Pharmaceutical Society of Japan
摘要：To investigate involvement of cellular glycosphingolipids in the propagation of influenza viruses in host cells, MDCK cells were treated with inhibitors for sphingolipid biosynthesis, fumonisin B1 and d , l - threo -1-phenyl-2-decanoylamino-3-morpholino-1-propanol. Continuous treatment of the cells with either inhibitor during pre- and post viral inoculation, but not the pretreatment alone, significantly reduced viral infection, but not viral attachment to the cells. Immunocytochemical analysis demonstrated that cellular distribution of hemagglutinin, a viral glycoprotein, was drastically altered when the cells were continuously treated with the inhibitors during pre- and post viral inoculation, but not the pretreatment alone. Our findings strongly suggest that cellular sphingolipids play important roles in the events after viral adsorption to the host cells.
关键词：influenza virus;metabolic inhibitor;sphingolipid;viral infection