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标题：Slow Acting Protein Extract from Fruit Pulp of Momordica charantia with Insulin Secretagogue and Insulinomimetic Activities
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作者：Sirintorn Yibchok-anun ; Sirichai Adisakwattana ; Cheng Yu Yao 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2006
卷号：29
期号：6
页码：1126-1131
DOI：10.1248/bpb.29.1126
出版社：The Pharmaceutical Society of Japan
摘要：The protein from Thai bitter gourd ( Momordica charantia ) fruit pulp was extracted and studied for its hypoglycemic effect. Subcutaneous administration of the protein extract (5, 10 mg/kg) significantly and markedly decreased plasma glucose concentrations in both normal and streptozotocin-induced diabetic rats in a dose-dependent manner. The onset of the protein extract-induced antihyperglycemia/hypoglycemia was observed at 4 and 6 h in diabetic and normal rats, respectively. This protein extract also raised plasma insulin concentrations by 2 fold 4 h following subcutaneous administration. In perfused rat pancreas, the protein extract (10 μg/ml) increased insulin secretion, but not glucagon secretion. The increase in insulin secretion was apparent within 5 min of administration and was persistent during 30 min of administration. Furthermore, the protein extract enhanced glucose uptake into C₂C₁₂ myocytes and 3T3-L1 adipocytes. Time course experiments performed in rat adipocytes revealed that M. charantia protein extract significantly increased glucose uptake after 4 and 6 h of incubation. Thus, the M. charantia protein extract, a slow acting chemical, exerted both insulin secretagogue and insulinomimetic activities to lower blood glucose concentrations in vivo .
关键词：Momordica charantia;antihyperglycemia;protein extract;pancreatic perfusion;insulin secretagogue;insulinomimetic