文章基本信息

标题：Effects of Overexpression and Antisense RNA Expression of Orf17, a MutT-Type Enzyme
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作者：Mika Hori ; Taketoshi Asanuma ; Osamu Inanami 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2006
卷号：29
期号：6
页码：1087-1091
DOI：10.1248/bpb.29.1087
出版社：The Pharmaceutical Society of Japan
摘要：The Escherichia coli Orf17 (NtpA, NudB) protein, a MutT-type enzyme, hydrolyzes oxidized deoxyribonucleotides, including 8-hydroxy-2′-deoxyadenosine 5′-triphosphate and 8-hydroxy-2′-deoxyguanosine 5′-triphosphate, in vitro . To examine its in vivo role(s) in bacteria, plasmid DNAs containing the orf17 gene in the sense and antisense orientations were introduced. When the Orf17 protein was overexpressed in mutT cells, the rpoB mutant frequency was decreased. On the other hand, similar effects were not observed when Orf17 was overexpressed in wild type and orf135 cells. Expression of the antisense RNA of the orf17 gene did not produce an obvious phenotype, such as increased mutant frequency and resistance to ionizing radiation. These results suggest that the role of the Orf17 protein is to back up the MutT function, and to assist in the elimination of 8-hydroxy-2′-deoxyguanosine nucleotides.
关键词：Orf17;MutT;8-hydroxy-dGTP;nucleotide pool sanitization;antisense RNA