文章基本信息

标题：Effect of 2-Methoxyestradiol, Buthionine Sulfoximine and Hydrogen Peroxide on the Viability of Renal Carcinoma Cell Lines (ACHN and ACVB)
本地全文：下载
作者：Yuka Hirai ; Noriko Kawabe ; Yoshiko Tsuda 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2006
卷号：29
期号：5
页码：1064-1067
DOI：10.1248/bpb.29.1064
出版社：The Pharmaceutical Society of Japan
摘要：2-Methoxyestradiol (2-ME), an endogenous metabolite of 17β-estradiol, induces the intracellular accumulation of superoxide anion (O₂^·−) and buthionine sulfoximine (BSO) is an inhibitor of glutathione (GSH) synthesis. We have examined the combination anticancer effect of 2-ME and BSO accompanied with hydrogen peroxide (H₂O₂). 2-ME inhibited cell growth in renal carcinoma cell lines (ACHN and ACVB) accompanied by an increase in the intracellular contents of GSH. The combination of 2-ME, BSO and H₂O₂ showed a significant antiproliferation effect in both ACHN and ACVB. The intracellular levels of reactive oxygen species (ROS) with a combination with 2-ME and H₂O₂ in ACHN and ACVB pretreated with BSO were markedly increased, which may have contributed to the potential antiproliferative action.
关键词：2-methoxyestradiol;reactive oxygen species;buthionine sulfoximine;renal carcinoma cell