文章基本信息

标题：Scavenging of Reactive Oxygen Species by Eriobotrya japonica Seed Extract
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作者：Junko Yokota ; Daisuke Takuma ; Atsuhide Hamada 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2006
卷号：29
期号：3
页码：467-471
DOI：10.1248/bpb.29.467
出版社：The Pharmaceutical Society of Japan
摘要：We have clarified that Eriobotrya japonica seed extract has strong antioxidative activity, and is effective for the prevention and treatment of various diseases, such as hepatopathy and nephropathy. In this study, to investigate the influences of components of Eriobotrya japonica seed extract on its antioxidative activity, extracts were prepared using various solvents ( n -hexane (Hex), ethyl acetate (EtOAc), n -butanol ( n -BuOH), methanol (MeOH) and H₂O) and the antioxidative activity of the solvent fractions and components was evaluated based on the scavenging of various radicals (DPPH and O₂⁻) measured by the ESR method and the inhibition of Fe³⁺-ADP induced NADPH dependent lipid peroxidation in rat liver microsomes. The radical scavenging activities and inhibitory activities on lipid peroxidation differed among the solvent fractions and components. In the n -BuOH, MeOH and H₂O fractions, radical scavenging activity and inhibitory activity on lipid peroxidation were high. In addition, these fractions contained abundant polyphenols, and the radical scavenging activity increased with the polyphenol content. In the low-polar Hex and EtOAc fractions, the radical scavenging activity was low, but the lipid peroxidation inhibition activity was high. These fractions contained β-sitosterol, and the inhibitory activity on lipid peroxidation was high. Based on these findings, the antioxidative activity of Eriobotrya japonica seed extract may be derived from many components involved in a complex mechanism, resulting in high activity.
关键词：Eriobotrya japonica;ESR;natural antioxidant;LPO