文章基本信息

标题：Intersimple Sequence Repeats (ISSR) Molecular Fingerprinting Markers for Authenticating Populations of Dendrobium officinale KIMURA et MIGO
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作者：Jie Shen ; Xiaoyu Ding ; Dongyang Liu 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2006
卷号：29
期号：3
页码：420-422
DOI：10.1248/bpb.29.420
出版社：The Pharmaceutical Society of Japan
摘要：Intersimple sequence repeats (ISSR) molecular fingerprinting markers have been employed to authenticate eight populations of Dendrobium officinale using 10 primers selected from 76 ISSR primers. A total of 127 DNA fragments were amplified, of which 115 were polymorphic (90.5% of all bands). Sixteen specific authentication markers have been found. To enhance the efficiency of authentication, ISSR fingerprinting codes have been constructed using six polymorphic bands for authenticating D. officinale populations. Eight wild populations of D. officinale have been authenticated accurately using ISSR.
关键词：intersimple sequence repeats;Dendrobium officinale;population;molecular authentication